Enhancing Paracetamol with Silver Metal Ions

Enhancing Paracetamol with Silver Metal Ions1- IntroductionThe paracetamol (Fig. 1) is one of the famous analgesic medicates which is commonly named as N-acetyl-4-amino-phenol. Paracetamol has an antipyretic medication with light toxicological side effect 1-7. The fluorometry 8, luminescence 9, electrochemical 10, nuclear magnetized resonance, mass spectroscopy 11 and liquid chromatography 12 are different analyses techniques used to determination of paracetamol do drugs in pure and pharmaceutical fashions. Paracetamol has a side effect as hepatotoxic in man and animals if it was taken with oer dose 13-17. Herein in this research paper, we aimed to enhance the efficiency of the drug in the presence of funds metal ions by formation of new interwoven. This complex was proven using spectroscopic and thermo gravimetric analyses.Fig. 1 Chemical structure paracetamol2- Experimental2-1- MaterialsReagents (paracetamol and silver(I) nitrate) are in analytical grade and used without furt her purification.2-2- InstrumentationsThe elemental analysis (%carbon, %hydrogen and % nitrogen) results were metric using CHN-2400 Perkin Elmer analyzer instrument. FT-IR spectra were scanned on Bruker FT-IR spectrophotometer within 4000-400 cm-1 region. The UV-vis. spectra of reactants and silver(I) complex were scanned using Perkin-Elmer Lambda 4B spectrophotometer in dimethyl sulphoxide solvent. The thermo gravimetric analyses TG/DTG were carried out in nitrogen atmosphere using Schimadzu TGA-50H thermal analyzer.2-3- synthetic thinkingThe 1 mmol of AgNO3 was dissolved in 20 mL distilled water and then mixed to 20 mL of methanolic solution of paracetamol with 1 mmol ratio under magnetic stirring. The pH of mixture was neutralized at 7-8 using diluted ammonium hydroxide solution. The mixture was refluxed at 60 C and left to evaporate tardily at room temperature. The precipitate was filtered off, washy with hot methanol and dried at 60 C.2-3- Microbiological investigationThe bio logical activity of silver(I) complex was tested against bacteria and fungi with more than one test organism. The organisms used in the present investigation including twain bacteria (B.subtilis Gram +ve), (E. coli Gram ve) and two fungi (genus Aspergillus niger and Aspergillus flarus). The results of microbiological investigations of bacterial and fungi against synthesized silver(I) complex were assessments.3- Results and Discussion3-1-Elementl analysesThe experimental data of %carbon, %hydrogen and %nitrogen are matched with the calculated values ( confuse 1). The elemental analyses results are support that NO3 ions not detected. The silver(I) complex is air stable with highly melting point ccc oC.Table 1Elemental analyses and physical results of Ag(I) complexAg2(para)M.wt (g/mol)%C%H%N%M (1. cm2.mol-1)FoundCalcd.FoundCalcd.FoundCalcd.FoundCalcd.364.8827.026.331.871.934.003.8458.5059.127.03-2- Molar conductivityThe molar conductance value of the silver(I) complex of paracetamol drug in DMSO solvent with concentration of 1.0010-3 M was found to be 7.00 (1. cm2.mol-1) at 25 C. This data suggested that silver(I) complex is non-electrolytes.3-3- Infrared spectraThe infrared assignments data of paracetamol free drug and its silver(I) complex are tabulated in Table 2 and silver(I) complex is shown in Fig. 2. Based on the comparison between the infrared spectra of free paracetamol drug ligand and its silver(I) complex, it has been discussed that, the absorption band at 3300 cm-1 and 3200 cm-1 of free paracetamol stimulate been assigned to OH and NH stretching vibration motions. These bands have been absence in the spectra of the silver(I) complex due to the involvement in complexation. The strong-to-medium absorption bands at 1650 and 1260 cm-1 in the spectrum of para drug are assigned to the stretching vibration bands of (C=O) and (C-O), respectively. These bands are shifted to lower wavenumbers at 1627 and 1100 cm-1 due to sharing in coordination mode. The pr esence of new absorption band at 510 cm-1 in silver(I) complex is assigned to (Ag -O) stretching vibration. Upon the infrared spectral assignments, the silver(I) ions coordinated to para drug through two place of coordination (deprotonated of OH) and (deprotonated of NH and oxygen of carbonyl group) as shown in Fig. 3.Fig. 2 FT-IR spectrum of Ag(I) complex.Table 2 Infrared frequencies within 4000-400 cm-1 of para and its Ag(I) complex.Compound(OH) + (NH)(C=O)(CNH) amide group(C-O) phenyl group(M-O)para3300, 3200165015601260Ag2(para)162715501100510Fig. 3 Suggested structure of Ag(I) complex.3-4- UV-vis. spectraThe formation of the Ag(I) complex was also confirmed by UV-vis. spectra in DMSO solvent within the 200-600 nm range. It can see that free para has two absorption bands at 300 and 390 nm due to -* intra-ligand transition of the aromatic ring and n-* electronic transition, respectively.3-5- Thermo gravimetric analysesThe heating rate was controlled at 10C/min under nitrogen envi ronment. The weight loss was scanned from room temperature till 1000 C. The thermo gravimetric curve of silver(I) complex is shown in Fig. 4. The thermal decomposition of Ag2(para) complex exhibit at two step. These steps are occurring at 200-1000 C and corresponding to the decomposition of para molecule with a weight loss of 11.4%. The final residual product is silver metal contaminated with few carbon atoms.Fig. (3) TG/DTG curve of Ag(I) complex of paracetamol.3-6- Microbiological investigationAntibacterial and antifungal activities of silver(I) paracetamol complex are assessed against Escherichia coli (G ve), Bacillus subtilis (G +ve) and antifungal (Aspergillus niger and Aspergillus flavus). The healthful activity scanned based on the size of inhibition zone. Ag(I) complex is found to has high activity against bacteria and fungi. The data listed in Table 3 and is shown in Fig. 4.Table 3 Antimicrobial data of para complexFig. 4 Statistical data of biological activities of DMSO c ontrol and silver(I) complexReferencesM.A. Jan Mens, Best Pract. Res. 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